Table 2 List of antibodies used for western blot
From: Impairing Gasdermin D-mediated pyroptosis is protective against retinal degeneration
Antibody | Dilution | Company | Catalogue number |
|---|---|---|---|
Rabbit Anti-Caspase-1 | 1:1000 | Cell Signaling | E2Z1C |
Mouse Anti-Caspase-1 | 1:1000 | Abcam, Cambridge, UK | ab138483 |
Rabbit anti GSDMD | 1:1000 | Abcam, Cambridge, UK | EPR19828 |
Rabbit anti GAPDH | 1:3000 | Sigma Aldrich, MO, USA | G9545 |
Goat anti Rabbit Horseradish peroxidase (HRP) conjugate | 1:1000 | Bio-Rad, CA, USA | 170–6515 |
Goat anti mouse Horseradish peroxidase (HRP) conjugate | 1:1000 | Bio-Rad, CA, USA | 170–6516 |