Fig. 1

Nr4a1^−/− and Nr4a1^se2/se2 mice are deficient in non-classical monocytes. A–H Gating strategy to include live cells (A), CD45⁺ cells (B), CD11b⁺Lin^neg cells (C), and CD64⁺ vs CD64^neg cells (D). Lineage (Lin) gate included T cells, B cells, NK cells, Eosinophils, and Neutrophils. E Gating of microglia vs macrophages from CD64⁺ cells. F. Gating of macrophage subtypes. G Gating of DCs from CD64^neg cells. H Gating of monocyte subtypes from non-DCs. I–K Representative monocyte plots from control and laser-treated WT (I) Nr4a1^−/− (J), and Nr4a1^se2/se2 (K) eyes. L, M Number of classical monocytes from WT vs Nr4a1^−/− eyes (L) and WT and Nr4a1^se2/se2 eyes (M). N: Numbers of non-classical monocytes from WT, Nr4a1^−/−, and Nr4a1^se2/se2 eyes. N = 7–12 mice per group. Data were analyzed using Brown–Forsythe and Welch ANOVA followed by Dunnett’s T3 multiple comparisons test (L, M) or two-way ANOVA followed by Sidak’s multiple comparisons test (N) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. DC dendritic cell, Mono monocyte, WT wildtype