Fig. 5

Multimodal sequencing of CD45⁺ immune cells. A Experimental overview. Retina/RPE/choroid/sclera was isolated from the four experimental groups before CD45⁺ FACS sorting and subsequent multimodal (RNA + surface protein) sequencing. B Weighted nearest neighbor (WNN) integration of RNA and ADT assays was performed before dimensionality reduction with uniform manifold approximation and projection (UMAP). C Canonical marker genes are displayed for each cellular cluster in a dot plot. D The contribution of the experimental conditions to each cluster is visualized in the faceted wnnUMAP plot. E The contribution of the four experimental groups to each cluster is visualized with a stacked bar chart. Mac macrophage, C-mono classical monocyte, NC-mono non-classical monocyte, DC dendritic cell, PMN polymorphonuclear neutrophil, NK-cell natural killer cell