Fig. 4

Pathway enrichment analysis of the effect of brain injury in WT and IL-1R1 gKO mice. The number of significant differentially expressed genes (DEGs) within their respective functional groups, shown as upregulated (red bars) and downregulated (blue bars), were determined based on annotations from the NanoString Neuroinflammatory panel. Genes were permitted to overlap across multiple functional groups. To evaluate whether the total number of significant DEGs within each functional category differed significantly from chance, we employed a binomial approach, calculating the probability that the total number of significant DEGs per category resulted in significant changes for that functional group (*p < 0.05). Gray boxes indicate functional gene groups of interest, including astrocyte, microglial, and neuronal function