Fig. 6

IL-1’s effect on select genes from Astrocyte, Microglia, and Neuron/Neurotransmission functional pathways at 3 h, 9 h, 24 h, and 72 h following a CHI in mice. The first (top) row displays four genes correlated with astrocyte function (Amigo2, Fkbp5, Lcn2, and Slc6a1). The second (middle) row highlights four genes connected with microglia function (CD68, P2ry12, Tmem64, and Trem2). The third (bottom) row shows four genes associated with neuron and neurotransmission function (Bdnf, Dlg1, Grm3, and Homer1). White groups indicate wild-type genotype, whereas yellow groups indicate IL-1R1 gKO genotype. Sham groups are combined of each respective genotype sham (WT sham or IL-1R1 gKO sham) at 3-, 9-, 24-, and 72-h post-sham procedure. Y-axis displays Z-score data for gene expression. X-axis indicates genotype and injury groups. Grey line indicates the reference line of the mean Z-score for combined WT and IL-1R1 gKO sham groups. To compare genes with significant effects in the two-way ANOVA (Table 1), post hoc tests were applied. A Dunnett's multiple comparison test was used to compare 3 h, 9 h, 24 h, and 72 h to the sham control group (^†p < 0.05, ^††p < 0.01, ^†††p = 0.001, ^††††p = 0.0001). The effect of genotype at each time point was compared using an unpaired two-tailed t-test (*p < 0.05, **p < 0.01, ***p = 0.001, ****p =  < 0.0001)