Fig. 3
Endothelial UTX knockout diminished BSCB permeability post-OGD exposure. a, b FITC-dextran transports assay the permeability of UTXf/f and UTX−/− SCMECs at pre- and post-OGD. n = 3 per group. c Representative immunofluorescence images of TJs-related protein (Claudin-5, Occludin, and ZO-1) in the UTXf/f and UTX−/− SCMECs when exposed to OGD. Scale bar, 20 μm. d Quantitative evaluation of the fluorescence intensity of Claudin-5, Occludin, and ZO-1 in c. n = 6 per group. e Quantitative evaluation of intercellular distance in c. n = 6 per group. f–h qRT-PCR verification of the mRNA levels of Claudin-5, Occludin, and ZO-1 in the UTXf/f and UTX−/− SCMECs when exposed to OGD. n = 3 per group. Data are represented as mean ± SEM. ns P > 0.05, *P < 0.05, **P < 0.01