Fig. 6
MLCK is integral to EC permeability modulation following in vitro OGD treatment. a, b FITC-dextran transports assay the permeability of UTXf/f, UTX−/−, UTX−/− + LV CON, and UTX−/− + LV MLCK-OE SCMECs at pre- and post-OGD. n = 3 per group. c Representative immunofluorescence images of TJs-related protein (Claudin-5, Occludin, and ZO-1) in the UTXf/f, UTX−/−, UTX−/− + LV CON, and UTX−/− + LV MLCK-OE SCMECs when exposed to OGD. Scale bar, 20 μm. d Quantitative evaluation of the fluorescence intensity of Claudin-5, Occludin and ZO-1 in c. n = 6 per group. e Quantitative evaluation of intercellular distance in c. n = 6 per group. f Western blotting analysis of the TJs-related protein levels including ZO-1, Occludin, and Claudin-5 in UTXf/f, UTX−/−, UTX−/− + LV CON, and UTX−/− + LV MLCK-OE SCMECs after OGD. g Quantitative analysis of the expression levels of ZO-1, Occludin, and Claudin-5 in f. n = 3 per group. Data are represented as mean ± SEM. ns P > 0.05, **P < 0.01