Fig. 2
From: β-adrenergic signaling triggers enteric glial reactivity and acute enteric gliosis during surgery
Acute enteric gliosis is modulated by sympathetic innervation. A Analysis of enriched GO-terms in mRNA from Sox10iCreERT2/Rpl22HA/+enteric glia 3 h after IM for POI hallmarks related to gliosis triggering pathways. B Heat map for the GO-term "GPCR signaling" in naïve and IM3h samples of mRNA from Sox10iCreERT2/Rpl22HA/+ enteric glia and with selected genes highlighted. C Schematic description of chemical sympathetic denervation of C57BL6 mice with three consecutive intraperitoneal 6-OHDA injections (days 1–3). After 14 days, mice underwent IM. ME was isolated three hours later (IM3h) for qPCR and RNA sequencing. D Confocal images of immunohistological stainings of TUBB3 (magenta) and TH (green) expression in whole mounts of control (saline) and 6-OHDA treated (sympathectomized/STX) small bowel ME 17 days after injection. (n = 6 animals per condition). Scale bar (100 µm). E qPCR analysis showing fold changes of mRNA levels (mean ± SEM) from IM3h/Saline and IM3h/STX mice for enteric gliosis-related genes (2−ΔΔCT, 18S, IM3h + saline; n = 6 animals per condition; Student’s t-test, * < 0.05, ** < 0.01). F Analysis of enriched POI hallmark GO-terms in IM3h/Saline total RNA and comparatively reduced in IM3h/STX samples related to inflammatory response and migration. G RNA-Seq heat map of our “acute enteric gliosis” GO-term for naïve and IM3h samples treated with saline or 6-OHDA (STX), and an indication of STX-affected genes (black line)