Fig. 2From: Single-cell RNA sequencing unveils Lrg1's role in cerebral ischemia‒reperfusion injury by modulating various cellsEffects of Lrg1 knockout on brain tissue damage in cerebral ischemia‒reperfusion mice. Mice were exposed to 1 h of ischemia and 24 h of reperfusion. A, B Gross slice figures of MCAO/R mouse brains stained with 2,3,5‒triphenyl tetrazolium chloride. The brain appeared red based on the interaction between TTC and dehydrogenase in the no infarction area, and the color faded to white in the no infarction area. Error bar graph showing the results of TTC staining in different groups. Data are expressed as the mean ± SD, n = 8. C, D Gross appearance of brains exposed to 1 h of ischemia and 24 h of reperfusion was observed based on Evans blue staining. Error bar graph showing the results of Evans blue staining in different groups. Data are expressed as the mean ± SD, n = 8. E H&E staining reveals morphology of brain tissues of MCAO/R mice based on light microscopic assessment. The damaged brain tissues exhibited white interspaces, pyknotic nuclei, appeared holes, and signs of bleeding. Scale bar = 1 × ; 200 × ; 400 × . F Measurement of neurological deficit scores of MCAO/R mice. Error bar graph showing the results of neurological deficit score in different groups. Data are expressed as the mean ± SD, n = 8Back to article page