Fig. 4

CTSS cleaves Ephrin-B2. A Blot of full-length Ephrin-B2 (fEphrin-B2) and cleaved Ephrin-B2 (cEphrin-B2) at the site of IAN injury at 14 day post-IANX. B, C Columns represent the average expression values of fEphrin-B2/β-actin (B) or cEphrin-B2/β-actin (C). n = 5 in each, one-way ANOVA post hoc Tukey’s test. *P < 0.05, ***P < 0.001 in B, Kruskal–Wallis post hoc Dunn’s test, *P < 0.05 in C. D Blot of fEphrin-B2 and cEphrin-B2 after mixing of recombinant human Ephrin-B2 (rhEphrin-B2) and recombinant human CTSS (rhCTSS). E Column represents the average values of the integrated density of fEphrin-B2 or cEphrin-B2. n = 4 independent experiments, one-way ANOVA post hoc Tukey’s test. *P < 0.05, **P < 0.01, ***P < 0.001. Data represent the mean ± SEM in B, C, and E. All data points are shown as open circles