Fig. 6

CTSS induces c-Jun in Schwann cells via the shedding of Ephrin-B2 on fibroblasts. A Schematic illustration of the isolation of Schwann cells (SCs) and fibroblasts (Fb) from the trigeminal nerve. B Images showing S100β-positive cells or αSMA-positive cells from isolated cells. Pie charts show the purity of S100β-positive cells or αSMA-positive cells isolated from mixed cultures of cells from the trigeminal nerve. n = 6 independent culture. C Images show c-Jun and Hoechst immunofluorescence in cultured SCs following treatment with rhCTSS, rhCTSS + Z-FL, or rhCTSS + Ephrin-B2 blocking peptide (EBP). D Column represents the average ratio of c-Jun-positive cells per SCs culture. n = 6 independent cultures, one-way ANOVA post hoc Tukey’s test. **P < 0.05, ***P < 0.01. All data points are shown in open circles