Fig. 7

Cfp deficiency in macrophages and neutrophils attenuates inflammation and protects against ischemic brain injury. a Gating strategy for microglia based on CD45^int and CD11b⁺. b-d qPCR assessment of Il1b, Tnf and Il6 expression levels in microglia isolated from mice 1 d and 3 d after tMCAO. n = 6 mice per group, *P < 0.05 versus the Cfp^fl/fl group, unpaired Student’s t test. e–g Evaluation of neurological function between LysM-cre Cfp^fl/fl and Cfp^fl/fl mice according to mNSS score (e), grip strength (f) and rotarod test (g) at 1 d and 3 d after tMCAO. n = 10 mice per group, *P < 0.05 versus the Cfp^fl/fl group, **P < 0.01 versus the Cfp^fl/fl group, ***P < 0.001 versus the Cfp^fl/fl group, two-way ANOVA with Sidak's multiple comparisons test. h Representative images and quantification of the infarct area as assessed by TTC staining 1 d and 3 d after tMCAO. n = 8 mice per group, *P < 0.05 versus the Cfp^fl/fl group, unpaired Student’s t test