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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Naïve Huntington’s disease microglia mount a normal response to inflammatory stimuli but display a partially impaired development of innate immune tolerance that can be counteracted by ganglioside GM1

Fig. 3

Q7/7 and Q140/140 microglia display similar kinetics of recovery after LPS stimulation. Q7/7 and Q140/140 microglia were pre-incubated with LPS (100 ng/ml) for 12 h, washed and further incubated in serum-free medium for 6, 24 and 48 h. Expression of Il-1b, Il-6 and Tnf (A, N ≥ 3) and levels of TNF secreted into the medium (B, N ≥ 4) were measured at the end of the incubation with LPS (12 h LPS) and during the recovery phase without LPS. mRNA levels were normalized over the housekeeping gene Ppia (encoding cyclophilin A). One-way ANOVA was used to confirm the effect of time on microglia phenotype of each genotype. An unpaired two-tail t-test was used to compare genotypes at each time-point. Bars are mean values ± STDEV. *p < 0.05, **p < 0.01, ****p < 0.0001

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Journal of Neuroinflammation

ISSN: 1742-2094