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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Naïve Huntington’s disease microglia mount a normal response to inflammatory stimuli but display a partially impaired development of innate immune tolerance that can be counteracted by ganglioside GM1

Fig. 5

In vivo development of tolerance is partially impaired or delayed in the brain of Q140/140 mice. A Schematic experimental design. Q7/7 and Q140/140 mice received daily intraperitoneal injections of saline or LPS (0.5 mg/kg) for 4 consecutive days, according to the indicated scheme. Two injections of LPS (2X LPS) on consecutive days were used to trigger inflammation in the brain. Three or four injections of LPS were performed to induce tolerance. B Cytokines in brain cortex homogenates were measured with a Luminex® multiplex assay. In tolerized (3X LPS) Q140/140 mice, the levels of several pro-inflammatory cytokines were significantly higher than in Q7/7 mice. A fourth LPS injection was needed to lower Q140/140 cytokine levels to Q7/7 levels. N ≥ 4. Two-way ANOVA with Sidak’s multiple comparisons test. *, p < 0.05; **, p < 0.01; ***, p < 0.001

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Journal of Neuroinflammation

ISSN: 1742-2094