Fig. 6

35DPTA7 and 35DPTSA12 reduced the neurotoxicity of activated microglia. Primary microglia were plated on permeable membranes in basket-type cell-culture inserts. Some cultures were treated with the indicated concentrations of 35DBTA7 or 35DBTSA12, followed immediately by application of LPS (100 ng/ml); some cultures were untreated and some received LPS alone. After 1 h, the inserts were placed into wells containing primary cortical neurons. After an additional 18 h, neuronal survival was determined by MTT assay. Values represent the percentage of the values obtained in wells containing untreated microglia (black bar); the y-intercept represents the neuronal viability values obtained in the presence of microglia treated with LPS alone. Values are mean ± SEM of quadruplicate cultures. ****P < 0.0001 for all concentrations of 35DBTA7 vs. LPS alone; *P = 0.039 for 30 μM 35DBTSA12 vs. LPS alone (ANOVA and Dunnett post hoc)