Fig. 3

GPR120 agonism attenuates nucleus accumbens microglial reactivity. A GPR120 (red) expression in NAc microglia (Tmem119, green) detected by RNAscope in situ hybridization in adult mice. Scale bar, 20 µm. B The effect of IL-1β and/or TNF-α application on Iba-1, TNF-α, and IL-1β mRNA expression in primary microglia derived from NAc. C Time course of cytokine -induced proinflammatory and microglial marker mRNA expression. Effect of GPR120 agonist pre-treatment on cytokine-induced D Iba-1, E TNF-α, F IL-1β, G IL-6, and H Ccl2 mRNA expression (n = 4). I Representative images of NAc microglia in slice preparations derived from mice ICV-injected with the TNF-α and IL-1β mixture with/without ICV CpdA pre-treatment. Scale bar, 20 µm. J Number of Iba-1 positive cells (n = 3). K Iba-1 intensity, L cell area, M cell perimeter, N circularity, O roundness, and P branches (n = 41–47 microglia/n = 3 brain). Mean ± SEM; one-way ANOVA with post hoc Sidak multiple comparison test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 vs Veh