Fig. 2

Intra-vitreal delivery of rAAV–sFKN prevents neuronal cell loss and microgliosis in the diabetic retina. A Confocal images of retinal tissues stained for NeuN (red) and Iba1 (green), with representation of cellular tracing for transformation index analysis (Inset) (Scale bar; 100 µm). B, C Quantification of retinal immunofluorescence analysis of NeuN⁺ cells/mm³ (B) and Iba1⁺ cells/mm³ (C). Data are shown as mean ± SD, n = 4–11 mice per group, where each dot represents an individual mouse across six experiments. D Transformation index quantification, where each dot represents an individual microglia cell n = 50–60 per group. Data shown as mean ± SD. *p < 0.05, **p < 0.01, and ****p < 0.0001 using Student’s t test, Welch’s correction