27-hydroxycholesterol causes cognitive deficits by disturbing Th17/Treg balance and the related immune responses in mild cognitive impairment patients and C57BL/6J mice

Table 1 Primers used in this study

Primer	Forward sequence (5'-3')	Reverse sequence (5'-3')
RORγt	ACAAATTGAAGTGATCCCTTGC	GGAGTAGGCCACATTACACTG
Foxp3	TTTCACCTATGCCACCCTTATC	CATGCGAGTAAACCAATGGTAG
IL-17A	GAGCTTCATCTGTGTCTCTGAT	GCCAAGGGAGTTAAAGACTTTG
GM-CSF	TTCAAGAAGCTAACATGTGTGC	GGTAACTTGTGTTTCACAGTCC
MIP-3α	TCTTCCTTCCAGAGCTATTGTG	GACTGCTTGTCCTTCAATGATC
IL-10	TTCTTTCAAACAAAGGACCAGC	GCAACCCAAGTAACCCTTAAAG
TGF-β1	CCAGATCCTGTCCAAACTAAGG	CTCTTTAGCATAGTAGTCCGCT
IFN-λ2	GGATTGCCACATTGCTCAGTTCAAG	GTCCTTCTCAAGCAGCCTCTTCTC
CYP27A1	ATCGCACAAGGAGAGCAATGGTAC	GGCAAGGTGGTAGAGAAGATGAGC
CYP7B1	AACCCTTTCCAGTACCAGTATG	GTGAACGTCTTCATTAAGGTCG
APP	TGAATGTGCAGAATGGAAAGTG	AACTAGGCAACGGTAAGGAATC
SAA	ACACTGACATGAAGGAAGCTAA	CCTTTGAGCAGCATCATAGTTC
GAPDH	GGTTGTCTCCTGCGACTTCA	TGGTCCAGGGTTTCTTACTCC

RORγt: retinoic acid-related orphan receptor γt; Foxp3: forkhead box protein p3; IL: Interleukin; GM-CSF: granulocyte–macrophage colony-stimulating factor; MIP-3α: macrophage inflammatory protein 3α; TGF-β1: transforming growth factor β1; IFN-λ2: Interferon-λ2; CYP27A1: sterol 27-hydroxylase; CYP7B1: oxysterol 7alpha-hydroxylase; APP: amyloid precursor protein; SAA: serum amyloid A

ISSN: 1742-2094