Fig. 3

Leukocyte–endothelium interactions measured in vivo increased following stroke and were reduced by iNO treatment. A Example images of rolling leukocytes along the wall of cerebral venules measured 4 h after sham surgery (left), MCAo (center), and MCAo followed by iNO treatment (right); leukocytes and vessels were stained with rhodamine 6G (magenta) and FITC-dextran (cyan), respectively. The direction of blood flow is indicated by the arrow, and scale bars represent 10 μm. B Summary of the number of rolling leukocytes measured in the cerebrovascular endothelium. C Representative images of adhered leukocytes (magenta) and the cerebral vasculature (cyan); arrows indicate stalled leukocytes, and scale bars represent 50 μm. D Quantification of the total number of leukocytes adhered to the endothelium in the indicated groups. E Flow cytometry gating strategy used to analyze the immune cells in the blood samples. F Quantification of Ly6G-high (neutrophils) and Ly6C-high (monocytes) cells in the indicated groups. G The mRNA levels of the indicated selectins and integrin ligands were measured in the ipsilateral cerebral cortex in the indicated groups and are expressed relative to the corresponding contralateral hemisphere. *p < 0.05, **p < 0.01, and ***p < 0.001; Kruskal–Wallis (B p: 0.0006, F: p: 0.006 (Neutrophils) and G p: 0.0017 and 0.0005 (selectins)) or one-way ANOVA (D, F (Monocytes) and G (integrin ligands)); n = 5–10 per group