Fig. 2

Genetic knockdown MPO expression in BV2 cells decreased HOCl production and attenuated apoptosis in PC12 cells under co-cultured conditions with OGD/R exposure. In the transwell system, PC12 cells were co-cultured with BV2 cells transfected with non-targeted shRNA (Ctrl-shRNA) or MPO-targeted shRNA (MPO-shRNA). Transwell-OGD/R: BV2 and PC12 co-cultured cells were exposed to 4 h OGD with 24 h of reoxygenation. A, B Western blot analysis for MPO expression in BV2 cells transfected Ctrl-shRNA or MPO-shRNA after OGD/R stimulation. C, D Annexin V-FITC/PI staining flow cytometry assay for apoptotic cell death. E, F Western blot analysis for MPO expression in PC12 cells among the groups. G HKOCl-3 staining HOCl production from PC12 cells among the groups, bar = 200 μm. *p < 0.05, **p < 0.01, ****p < 0.0001, #p < 0.05, ####p < 0.0001. All data are presented as mean ± SEM. (Statistical methods: B, C, F one-way ANOVA followed by Tukey’s multiple comparisons test.)