Fig. 5

Dynamic changes and localization of MPO expression and HOCl production in ischemic brains during cerebral ischemia–reperfusion injury. Rats were subjected to 2 h of MCAO ischemia alone (I/R-0 h) or 2 h of MCAO ischemia plus 2, 4, 6, 24 h of reperfusion, termed as I/R-2 h, I/R-4 h, 1/R-6 h and I/R-24 h, respectively. MPO expression and HOCl production were detected by western blot analysis and HKOCl-3 staining fluorescence. A MPO expression in brain tissues; B HOCl level in rat brains. Bar = 500 μm. C Quantitative results of HOCl level in rat brains. D Pearson correlation coefficients for correlation between plasma HOCl level and mNSS in rat MCAO I/R injury. N = 16, r = 0.8694, p < 0.001. E Fluorescent imaging of MPO expression in microglia, neuron, and microvascular endothelial cells of the ischemic cortical area by co-stained with Iba-1, NeuN or vWF, respectively. Nucleus was stained with DAPI, bar = 10 μm. F HKOCl-3 staining fluorescent imaging for HOCl production in microglia, neuron, and microvascular endothelial cells co-stained with Iba-1, NeuN or vWF, respectively, nucleus was stained with DAPI, bar = 10 μm. *p < 0.05, **p < 0.01. All data are presented as mean ± SEM. (Statistical methods: A, C one-way ANOVA followed by Tukey’s multiple comparisons test; D Pearson correlation coefficient.)