Skip to main content
Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Hypochlorous acid derived from microglial myeloperoxidase could mediate high-mobility group box 1 release from neurons to amplify brain damage in cerebral ischemia–reperfusion injury

Fig. 6

MPO inhibitor 4-ABAH reduced MPO/HOCl production, attenuated infarct volume and BBB disruption, and improved neurological functions in rat brains after MCAO ischemia–reperfusion (I/R). I/R rats were subjected to 2 h of MCAO ischemia plus 24 h of reperfusion. Sham control rats were subjected to similar operation without MCAO. MPO inhibitor 4-ABAH (20 mg/kg wt.) was intraperitoneally injected at the onset and 12 h of reperfusion, respectively (A) Western blot analysis for MPO expression. B, C HOCl level in I/R brains. D, G TTC staining and quantitative analysis showing infarct volumes in sham control and I-2 h/R-24 h rat brains with or without 4-ABAH treatment. E, H Evans blue leakage in rat brains of sham control and I-2 h/R-24 h with or without 4-ABAH treatment. F Neurological deficit scores (mNSS) in I-2/R-24 h rats with or without 4-ABAH treatment. *p < 0.05, **p < 0.01. All data are presented as mean ± SEM. (Statistical methods: A, C, H one-way ANOVA followed by Tukey’s multiple comparisons test; F, G two-tailed t-test.)

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094