Fig. 6

Identification and count of Purkinje cells in the cerebellum of PCD mice at P30. A–C Immunolabeling of Purkinje cells with CB (green); nuclei were counterstained with DAPI (purple). Note how the surviving Purkinje cells are mainly located in lobe X at P30 (C). D Chart showing the linear density of Purkinje cells both in the whole vermis and only in lobe X. Note the significant reduction in the density of Purkinje cells in lobe X of sham-operated animals, as well as the neuroprotective effect achieved by transplanting IMCs. Data are expressed as mean ± SEM. *p < 0.05. Scale bar: 200 μm (A), 50 μm (B) y 1 mm (C)