Fig. 6

Quantitative real-time PCR of chemokines and surface receptors. A Peritoneal macrophages were isolated from WT and α7nAChR^−/− mice and subsequently incubated with LPS (10ng/mL) and PNU (30μM) overnight before isolation of RNA and production of cDNA. Plots show relative mRNA levels of CCR2 and its ligand MCP-1, and CCR5 and its ligand RANTES. B Cells were prepared for qRT-PCR identically to part A. Plots showing relative mRNA levels of integrin subunits αM, αX, αD, and β1. Experiment had eight independent replicates. Data were analyzed using the Livak Method. Statistical analysis was performed with a student's t test. *P < 0.05, **P < 0.01