Cholinergic signaling via the α7 nicotinic acetylcholine receptor regulates the migration of monocyte-derived macrophages during acute inflammation

Table 1. Primers sequence used for qPCR.

Primer	Sequence (5’–3’)	References
αX forward	CTGGATAGCCTTTCTTCTGCT	[30]
αX reverse	GCACACTGTGTCCGAACTCA	[30]
αM forward	TCCGGTAGCATCAACAACAT	[31]
αM reverse	GGTGAAGTGAATCCGGAACT	[31]
αD forward	GGAACCGAATCAAGGTCAAGT	[32]
αD reverse	ATCCATTGAGAGAGCTGAGCTG	[32]
CCR2 forward	ACAGCTCAGGATTAACAGGGACTTG	[27]
CCR2 reverse	ACCACTTGCATGCACACATGAC	[27]
CCR5 forward	TCCGTTCCCCCTACAAGAGA	[28]
CCR5 reverse	TTGGCAGGGTGCTGACATAC	[28]
MCP-1 forward	TGGAGCATCCACGTGTTGGC	[33]
MCP-1 reverse	ACTACAGCTTCTTTGGGACA	[33]
RANTES forward	GCTTCCCTGTCATTGCTTGCTC	[34]
RANTES reverse	AGATGCCCATTTTCCCAGGACC	[34]
β1 forward	GTGACCCATTGCAAGGAGAAGGA	[35]
β1 reverse	GTCATGAATTATCATTAAAAGTTTCCA	[35]
GAPDH forward	AAGGTCATCCCAGAGCTGAA	[36]
GAPDH reverse	CTGCTTCACCACCTTCTTGA	[36]

ISSN: 1742-2094