Fig. 2

Dox treatment disrupts the gut microbiome in both Tg and WT mice. Analysis of gut microbiome from stool samples collected at 6 wk of age while half of each genotype was still receiving dox chow, focusing on the effect of dox exposure. A, B Observed OTUs (A) and Simpson index (B) reveal that dox treatment reduced α-diversity in both Tg and WT mice. C, D Principal coordinate analysis (PCoA) of weighted UniFrac distances indicate that dox altered β-diversity in both WT (C) and Tg mice (D). E The relative abundance of bacterial taxa was shifted by dox treatment in both genotypes. Statistical testing: Kruskal–Wallis (A, B), PERMANOVA (C, D), and Mann–Whitney U, reporting FDR-adjusted p-value (E). n = 5–7 mice/group. *p > 0.05, **p > 0.01. Red and blue = dox-treated Tg and WT, respectively; grey and white = untreated Tg and WT, respectively