Fig. 4From: IKKβ deletion from CNS macrophages increases neuronal excitability and accelerates the onset of EAE, while from peripheral macrophages reduces disease severityKnocking out IKKβ selectively from CNS macrophages results in early demyelination and neuroinflammation in EAE. A Specimen images of paraffin-embedded coronal spinal cord sections from IKKβF/F and ΜgΙΚΚβKO mice stained with LFB (left column) and H/E (right column) showing the evolution of demyelination and immune cell infiltration, respectively, during MOG35-55-induced EAE. Specifically, in the naïve spinal cord, at a pre-onset stage (dpi 8), at the onset (of the ΜgΙΚΚβKO mice), at the peak and at the chronic phase of the disease. B Semi-quantification of the mean demyelination level in spinal cord of IKKβF/F and ΜgΙΚΚβKO mice shown in A, left column. C Semi-quantification of the mean immune cell infiltration level in spinal cord of IKKβF/F and ΜgΙΚΚβKO mice shown in A, right column. Numbers of mice are annotated as scatter dots on the bars. All mice were adult females 2–4 months oldBack to article page