Fig. 3

CD44 receptor is upregulated in microglia after ischemic stroke. A The FPKM of Cd44 according to the bulk-RNA seq data from our previous work. B Representative immunoblot image showing the protein level of CD44 from ischemic brain tissue at different time points (Sham, 1d, 3d) after MCAO. GAPDH served as the loading control. C, D Flow cytometry strategy labeling microglial CD44 (C) and the quantification of mean fluorescence intensity (MFI) of CD44 in microglia from ischemic brain tissue 1 day after MCAO (D). n = 4 mice per group. E Representative immunofluorescence images of CD44 expression in cortex neurons (NeuN⁺), astrocytes (GFAP⁺) and microglia (Iba1⁺) from mice after MCAO 1 day. n = 4 mice per group. Scale bar, 100 μm. F Quantification of co-localization of CD44 with NeuN, GFAP or Iba1 in cortex of mice 1 day after MCAO. G Representative immunofluorescence image of co-localization of Iba1 (red), SRGN (green) and CD44 (blue). Scale bar, 40 μm. H Co-immunoprecipitation analysis of SRGN and CD44. Data represented as mean ± SEM, * p < 0.05, ****p < 0.0001