Fig. 7

SRGN activates NF-κB p65 signaling pathway in microglia. A-E Representative immunoblot images (A) and quantification of p-p65/ p65 (B), p-IKB/ IKB (C), p-STAT3/ STAT3 (D) and p-ERK/ ERK (E) for primary microglia treated with rSRGN (50 ng/ mL) or control solvent for 6 h. β-Actin served as the loading control. n = 3 per group. F Representative immunofluorescence images showing the cellular localization of NFκB p65 (green) in primary microglia induced by rSRGN (50 ng/ mL), LPS (100 ng/ mL) or a combination of both for 3 h. Scale bar, 20 μm. G-I The qPCR analysis of Il1b (G), Il6 (H) and Tnf (I) mRNA levels in primary microglia treated with rSRGN (50 ng/ mL, 3 h), with or without the pretreatment of JSH-23 (30uM, 2 h). n = 3 per group. Data represented as mean ± SEM, * p < 0.05, ** p < 0.01, ***p < 0.001, ns, no significant