Fig. 2

Human fetal organotypic brain slices cultures support productive HSV-1 and HSV-2 infection. A Microscopy analysis of green fluorescent protein (GFP) expression as a marker of cells infected with GFP-expressing HSV-1 or HSV-2. White arrow heads indicate foci of initial infection. Images shown are fluorescent microscopy pictures of the same human fetal organotypic brain slice (hfOBSC) at 24, 48 and 72 h post-infection (hpi) and are representative of hfOBSC cultures of five subjects. Scale bar: 500 µm. B Quantification of HSV-1 and HSV-2 DNA loads in culture medium was quantified by Taqman quantitative real-time PCR (qPCR) as described previously [27] of representative hfOBSC cultures. Data are presented as mean viral DNA copies/mL ± SD of two independent subjects (blue and red color)