Fig. 3

HSV-1 and HSV-2 preferentially infect astrocytes and neurons in human fetal organotypic brain slice cultures. A, B Brain slices were infected with 100 plaque forming units per mL of HSV-1-GFP or HSV-2-GFP. At 72 h post-infection, brain slices were fixed and longitudinally sectioned. Triple immunofluorescence staining of brain slice sections using antibodies against GFP (green color) expressed by both HSV-1 and HSV-2 strains combined with (A) MAP2 (neurons; magenta color) and Iba1 (microglia; red color) or (B) GFAP (astrocytes; magenta color) and Olig2 (oligodendrocytes; red color). All staining combinations have been counterstained with Hoechst (nuclear stain; blue color). Scale bar: 10 µm and 20 µm (inserts). C Quantification of the in situ data obtained at three separate levels of brain slices generated from two subjects (filled circles and triangles). Percentage marker-positive cells of all virus-infected cells are provided