Fig. 6

HSV-1 and HSV-2 infection induces necroptosis in human fetal organotypic brain slice cultures. A In situ immunofluorescence analysis of programmed cell death in HSV- and mock-infected human brain slices cultured until 72 h post-infection. Consecutive brain slice sections were stained for the apoptosis marker cleaved caspase 3 (CC3; red color), the pyroptosis marker gasdermin D (GSDMD; green color), or the necroptosis marker phosphorylated MLKL (pMLKL; white color). Images are representative of three subjects. Hoechst, nuclear stain (blue) and scalebar = 50 µm. B Quantification of the stainings obtained for A. Data are shown as mean percentage of marker-positive cells ± SD of three subjects. Two-tailed Student’s t test: p > 0.05 (ns) and p ≤ 0.05 (*)