Fig. 2

Sepsis damages meningeal lymphatic vasculature morphology in aged mice. A Representative immunofluorescence images of meningeal whole-mounts with OVA-647 stained with Lyve-1. B Graph depicting the percentage area of Lyve-1 coverage in the meningeal lymphatic vasculature, n = 6. C OVA-647 coverage in the meningeal lymphatic vasculature, n = 6. D Representative dot and contour plots showing the gating strategy used to isolate meningeal LECs (CD45⁻CD31⁺ podoplanin⁺). E Relative mRNA levels of Prox1 and Foxc2 in meningeal LECs sorted from mice treated with Vehicle or LPS, n = 4. *P < 0.05, **P < 0.01. All data are expressed as the mean ± SD