Fig. 8
Canonical anti-inflammatory agents do not affect disease-relevant neuroinflammation. NanoString analysis of bulk tissue transcriptomes after treatment with anti-inflammatory agents. A Hierarchical clustering of full transcriptomes highlight a lack of a consistent pattern of reversal by any of the agents assessed. Pearson correlation matrix of transcriptomic data confirm lack of robust reversal of chronic neuroinflammatory state. B Analysis of genes that exhibit cell type-specific expression indicates general increases in microglia and astrocyte-associated transcription. C Heat maps of gene panels for specific domains of biology indicate modest effects on microglia, but either no effects or exacerbation of disease state with respect to gene expression related to astrocyte activation, inflammatory processes, and neurotransmitter receptors. Heat maps in this figure indicate Z-scores derived from Log2[Fold Change]. Z-scores were capped at ± 6 in Panel C to facilitate visualization