Fig. 3
Effects of maternal obesity on sphericity, volume and number density of astrocytes in the offspring: examples of GFAP staining of astrocytes in the hippocampus of offspring of control dams (A) and offspring of obese dams (B), scale bar = 20 µm; three-dimensional plots of astrocyte ellipsoid shape in offspring of control dams (C) and offspring of obese dams (D); the effect of maternal obesity on offspring astrocyte spherical shape (E); larger size of astrocyte soma (hypertrophy) in the hippocampus of offspring of obese dams’ group (F); a significant lower density of astrocytes in CA1.SR subregion of hippocampus of offspring of obese dams (G); *p < 0.05, *** p < 0.001, (n = 6/group)