Fig. 4

Effects of maternal obesity on astrocytes morphology in the hippocampus of offspring: examples of images of 3D reconstructed astrocyte in the CA1.SR subregion of hippocampus in offspring of control (A) and obese dams mice which includes more and longer branches (B); blue color indicates the shortest processes, and red color is an indicator of the longest astrocytic processes, scale bar = 20 μm; astrocyte morphology analysis indicated no significant difference in the length of astrocyte branches (C) but a significant higher number of branches (D). No difference in complexity of arborization of astrocytes branches (E) but an abnormal development of astrocyte branches in the hippocampus of offspring of obese dams as compared to offspring of control dams (F, G). *p < 0.05, **p < 0.01, ***p < 0.001, (n = 6/group)