Fig. 2

Monocyte population shift in blood following CCI injury and CL-LPM treatment. A Schematic representation of experimental timeline. B, C Flow cytometry analysis for the percentage (%) of CD45⁺/CD11b⁺ cell population that are Ly6G⁻, Ly6G⁻/CD115⁺, or Ly6G⁺ in CL-LPM or control-LPM treated naïve mice (0 dpi, B) as well as absolute numbers (C). D, E Flow cytometry analysis for the percentage (%) of myeloid population, including absolute numbers (F). G–J Flow cytometry gating strategy to select live/singlets, CD11b⁺/CD45⁺, and Ly6G^–/CD115⁺ monocytes. n = 5 per group. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.00001. Student’s t-test (B, C); one-way ANOVA with Bonferroni post hoc (D–F)