Fig. 3
Distinctive morphology and gene expression signature in circulating CD115 + /Ly6Chi monocytes of sham and CCI-injured mice pre-treated with control or Cl-LPM at 1dpi. A Representative contour plots of side (SSC-A) and forward (FSC-A) scattering of Ly6Chi monocytes from sham and CCI-injured mice pre-treated with control or CL-LPM at 1 dpi. B The mean size (FSC-A) of Ly6Chi monocytes is significantly increased in Cl-LPM or control LPM- treated CCI-injured mice compared to control sham cells. C The mean granularity (SSC-A) of Ly6Chi monocytes is significantly increased in Cl-LPM-treated CCI-injured mice compared to other groups. D Cell size of whole blood monocytes from Cl-LPM treated mice at 1 dpi. E, F Representative images of differential quick cytology. G–J Relative mRNA expression Ly6c and neutrophil-like gene Gfi1 (G), primary granules (H), anti-inflammatory (I), and monocytes TF (J) in circulating CL-LPM monocytes relative to control injured mice at 1 dpi. n = 5/group, *p < 0.05; **p < 0.01; ****p < 0.0001. One-way ANOVA with Bonferroni post hoc (B–D); t-test (G–J)