Fig. 4

Phenotypic shift of infiltrating immune cells and reduced inflammatory cytokines in the cortex of Cl-LPM injured mice. A, B Flow cytometry gating for CD45^hi/CD11b + cells and CD54^lo/CD11b + microglia (A) followed by Ly6G⁺ selection of neutrophils and Ly6G⁻ myeloid (B). C Percentage of CD11b⁺/CD45^hi infiltrating immune cells, CD11b⁺/CD45^hi/Ly6G⁺ neutrophils, and CD11b⁺/CD45^hi/Ly6G-myeloid/macrophages in the ipsilateral cortex of Cl-LPM or control CCI-injured mice at 1 dpi. D Representative gating of Ly6C and Ly6G expressing cells from CD11b⁺/CD45^hi infiltrating immune cells in the damaged cortex. E The percentage of CD11b⁺/CD45^hi/Ly6G⁺ and CD11b⁺/CD45^hi/Ly6G⁻ cells co-labeled as Ly6C^hi or Ly6C^lo expression. F Representative FSC-A/SSC-A gating to evaluate size and granularity of Ly6C^hi cells in the injured cortex. G Quantification for the size and granularity of CD11b + /CD45^hi/Ly6G-/Ly6C.^hi cell in the injured cortex at 1 dpi. H–K Cytokine Array protein quantification showing the top differentially expressed cytokines in the ipsilateral cortex at 1 dpi. M String analysis of the top differentially expressed cytokines. n = 5/group, *p < 0.05; **p < 0.01; ****p < 0.0001. t-test (C, E, G)