Fig. 7
Top DGEs and Gene Ontology using scRNAseq cluster analysis of control and Cl-LPM monocytes. A Heatmap of top 10 genes in each annotated cell cluster in control monocytes. B Cell trajectory analysis using pseudotime shows a hub in Cluster (C) 0. C Volcano plot of DGEs in C0, non-classical. D GO analysis shows migration, cell–cell adhesion, actin assembly, regulation of inflammatory response and TNF production as top biological processes in C0. E Top 5 UP and down regulated genes in C0 that show inverse expression compared to C4. F Heatmap of top 10 genes in each annotated cell cluster in Cl-LPM monocytes. G Cell trajectory analysis using pseudotime. H Volcano plot of DGEs in C0, non-classical. I GO analysis shows oxidative phosphorylation, aerobic respiration, etc., as top biological processes in C0. J Top 5 UP and down regulated genes in C0. K Analysis of top DGE in neutrophil-like monocytes, C9. L GO analysis for C0. M–O Transfer of peripheral-derived monocytes (PDMs) by i.v. injection immediately following CCI injury. P, Q Gross images of injured brain at 1dpi shows tissue protection in mice receiving Cl-LPM PDMs (R). n = 5–8 *p < 0.05; one-way ANOVA, Bonferroni post hoc. Scale = 500 um in M–O and 0.5 cm in P and Q