Fig. 5

RNA-seq analysis of DEGs in STHdhQ111 and Q7 cells in response to IFNγ/LPS treatment. A–D Volcano plot showing DEGs by pairwise comparisons across different conditions as indicated. E, F Venn diagram showing upregulated (E) and downregulated (F) DEGs (treatment vs. control) in STHdhQ7 and Q111 cells. G A simplified diagram illustrating Arg biosynthesis and metabolism with key proteins indicated. Created with BioRender.com. The insets a–g are bar graphs showing the quantification of each related gene transcript in TPM across different conditions. The TPM values were extracted from RNA-seq analysis (now deposited as GSE241325). *Indicates significance between Q111-C and Q7-C; ^#indicates significance between Q7-T and Q7-C; ^$indicates significance between Q111-T and Q111-C; ^&indicates significance between Q111-T and Q7-T. The significance (q < 0.05) was calculated using the R package DESeq2 between two different groups