Fig. 3

Expression of native and transgenic FH variants at transcript and protein levels. A Left, at 3 dpi, mRNA expression levels of regulatory CFH variants were already detectable in the Müller cell fraction, but at low levels. Right, after 14 days, a stronger expression of both FH1-4^19-20 and FH1-7^19-20 mRNA variants was observed in purified Müller cells. Bars represent the mean ± SEM of n = 3–4 biological replicates. Each data point is represented by a dot in the graph. Unpaired t-test: *P < 0.05. B IHC results from cryosections of control and post-ischemic retinas after AAV treatment delineating FH deposition and the localization of microglia/macrophages by a labeling for IBA1 at 3 and 14 dpi. FH, factor H. Scale bar, 50 µm