Fig. 5

Cell type-specific analysis of complement component expression in the murine retina 3 and 14 dpi. qPCR data are presented as negative delta Ct values, i.e. that high numbers represent higher transcript levels on a logarithmic scale. Neuronal, microglia and Müller cell fractions were analyzed for transcripts of (A) the central component C3, (B) complement components involved in activation Cfd and Cfb or (C) inhibition of the complement cascade Cfh and Cfi and (D) Cfp that is involved in the amplification of C3 cleavage. Each data point per biological replicate (equals the number of mice studied: n = 2–5) is represented by a dot in the graph. Unpaired t-test: *P < 0.05; **P < 0.01. (All statistical analyses are available in Additional file 1: Tables S1, S2, and S3.)