Fig. 7

Microglia response to AAV_FH treatments following ischemia. A The expression of Aif1 mRNA (also known as IBA1) in control and ischemic retinas after AAV treatment was determined by qPCR. At 14 dpi, Aif1 levels were lower in AAV_FH1-4^19-20 treated retinas compared with AAV_EGFP ischemic controls (Unpaired t-test: **P < 0.01). B Quantification of IBA1-positive microglia/macrophages was performed in the respective retinal layers to detect cellular migration in response to the tissue damage (unpaired t-test). C Representative microscopic images of IBA1 immunostaining on retinal sections at 14 dpi. D Representative microscopic images of IBA1 and CD68 co-immunostaining on retinal sections at 14 dpi. E Mean fluorescence intensities of CD68, a lysosomal marker upregulated in activated microglia/macrophages, were measured in IBA1 + cells. Ordinary One-Way-Anova with Tukey’s multiple comparison: *P < 0.05; ***P < 0.001; ****P 0.0001. Colored asterisks indicate comparison of cells from ischemic retinas at 14 dpi versus 3 dpi of the same treatment group (as indicated by the color of the asterisk). Each data point per microglial cell (from 3–4 retinas per group) is represented by a dot in the graph. A, B Each data point per biological replicate (n = 3–4) is represented by a dot in the graph