Table 1 Antibodies used for immunofluorescence and flow cytometry
Antibody | Concentration |
|---|---|
Immunofluorescence primaries | Â |
Rabbit anti-human CD45 (clone EP322Y, #AB40763, Abcam) | 1/200 |
Goat anti-mouse CD45 (polyclonal, #AF114, R&D Systems) | 1/400 |
Chicken anti-mouse GFAP (polyclonal, #AB4674, Abcam) | 1/400 |
Immunofluorescence secondaries and nuclear dye | Â |
Alexa Fluor® 488 Donkey Anti-Human IgG (Fcγ-specfic) (polyclonal, #709545098, Jackson ImmunoResearch) | 1/100 |
Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Chicken IgY (IgG) (H + L) (polyclonal, #703546155, Jackson ImmunoResearch) | 1/400 |
Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H + L) (polyclonal, #711546152, Jackson ImmunoResearch) | 1/400 |
Alexa Fluor® 647 AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H + L) (polyclonal, #JI711606152, ImmunoResearch) | 1/400 |
Alexa Fluor® 647 AffiniPure F(ab')2 Fragment Donkey Anti-Goat IgG (H + L) (polyclonal, #JI705606147, Jackson ImmunoResearch) | 1/400 |
Hoechst 33342 Trihydrochloride Trihydrate (H3570, Invitrogen) | 1/1000 |
Flow cytometry | Â |
Anti-human CD14 BV711 (clone MPɸ9, #563372, BD) | 1/200 |
Anti-human CD45 APC-Cy7 (clone 2D1, #368516, Biolegend) | 1/50 |
Anti-mouse CD45 PE (clone 30-F11, #553081, BD) | 1/100 |
Anti-mouse/human B220 AF700 (clone RA3-6B2, #103232, Biolegend) | 1/25 |
Anti-mouse/human CD11b BV786 (clone M1/70, #101,243, Biolegend) | 1/400 |
Propidium iodide (#P1304MP, Life Technologies) | 1/3200 |
Anti-Cy7 MicroBeads (#130–091-652, Miltenyi Biotec Australia) |  |