Fig. 2

Removal of α-synuclein autoantibodies from serum rescues neurotoxicity. (A) Schematic representation of removal of α-syn AAbs from serum. (B) Representative photomicrographs of MAP2 staining (green) and NmC-positive cells (red) in α-syn + NmC transduced cultures, either without treatment (untreated), or after 1 d of exposure to serum containing α-syn AAbs, or to depleted serum. (C) Percent NmC positive cells (relative to untreated cells) after 1 and 3 d of exposure to serum containing α-syn AAbs or depleted serum, in α-syn + NmC expressing cells. N = 4 biological and 8 technical replicates for (C). Statistics by one-way ANOVA with Tukey’s multiple comparisons test; statistical power (1-ß error probability) > 0.9 for all conditions. n.s. = not significant; *** = p < 0.001