Fig. 4

α-synuclein (auto)antibodies do not affect astrocyte survival in rat primary neuron-astrocyte co-cultures. (A) Percent GFAP-eGFP positive cells in α-syn + NmC transduced cultures before treatment (0 h) and after 6 and 24 h of treatment with serum containing α-syn AAbs, or with serum depleted of α-syn AAbs, or with an commercial α-syn Ab (ab138501). (B) Western blot for astrocyte marker (GFAP) and internal control (Vinculin) with lysates prepared from α-syn + NmC transduced cultures after 24 h of treatment. (C) Quantification of western blots as relative GFAP expression after normalization to Vinculin expression. N = 4–5 biological replicates for all conditions. Statistics by one-way ANOVA with Dunnett’s multiple comparisons test