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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury

Fig. 7

SPOCK2 interacts with MT1-MMP to regulate the activation of MMP-2 by the SPARC_EC domain. A PPI prediction website STRING showed potential PPI between SPOCK2 and MT1-MMP. B Double immunofluorescent staining showed colocalization of SPOCK2 and MT1-MMP in the rat spinal dorsal horn after CCI-induced NP (Scale 100 um). C Co-IP of SPOCK2 and endogenous MT1-MMP in HEK-293 T cells transfected with pCMV6-SPOCK2-myc-DDK. D Schematic diagram of five SPOCK2 variant plasmids. E Transfection efficiency of plasmids pCMV6-myc-DDK, pCMV6-SPOCK2-myc-DDK, pCMV6-ΔKAZAL-myc-DDK, pCMV6-ΔSPARC_EC-myc-DDK, and pCMV6-ΔΔ-myc-DDK in HEK-293 T cells. F Co-IP of SPOCK2 and endogenous MT1-MMP in HEK-293 T cells transfected with five SPOCK2 variants. G Increased expression of active-MMP-2 in HEK-293 T cells transfected with pCMV6-SPOCK2-myc-DDK and pCMV6-ΔKAZAL-myc-DDK plasmids (*P < 0.05 vs empty)

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Journal of Neuroinflammation

ISSN: 1742-2094