Fig. 3

Tyrobp deletion prevents increased CD68 levels and reduction of PSD-95 in Q175 mice. A Representative images of CD68 immunofluorescent staining in the striatum from 10-month old WT and Q175 mice with (left) and without (right) Tyrobp. Scale bar: 10 µm. B Quantification of CD68-immunopositive area in the striatum (n = 4 mice per group with an average of 3 slices per mouse). C RT-qPCR of CD68 mRNA in the striatum in the same mice as in Fig. 2A with n = 6 mice per group. D Western blot and densitometric analysis of synaptophysin and PSD-95 proteins in striatal samples. n = 6 mice per group. (E) Representative confocal images showing PSD-95 (green) immunopositive clusters in the dorsal striatum of WT and Q175 mice with and without Tyrobp. Quantitative analysis is shown as mean ± SEM (n = 4 animals per group). Statistical analysis was performed using Two-Way ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001. Scale bars, 10 µm