Fig. 1

Cebpd/CEBPD is highly expressed in both mouse and human primary astrocytes after ouabain treatment. A CEBPD is highly expressed in the astrocytes of CA1 region in ouabain-treated mice. Immunofluorescence assays revealed that CEBPD (red) is predominantly expressed in astrocytes stained with GFAP (green) in mice subjected to ouabain treatment. B Western blot analysis showed an increase in CEBPD expression in human primary astrocytes treated with various doses of ouabain. C Quantitative data from (B) revealed an enhanced intensity of CEBPD in human primary astrocytes. The CEBPD was normalized using α-Tubulin. The quantitative data is presented as means ± SEM; N = 3; analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test, **p < 0.01. D Time-dependent treatment of astrocytes with 20 μM ouabain demonstrated an increase in CEBPD expression. E Quantitative data from (D) displayed the expression levels of CEBPD in astrocytes. The CEBPD was normalized using α-Tubulin. F Dose-dependent and G time-dependent treatments of astrocytes with ouabain revealed an increase in CEBPD transcription. Q-PCR was conducted to measure CEBPD mRNA levels. Quantitative data are presented as means ± SEM; N = 3; **p < 0.01